Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep
نویسندگان
چکیده
منابع مشابه
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
determination of maximal singularity free zones in the workspace of parallel manipulator
due to the limiting workspace of parallel manipulator and regarding to finding the trajectory planning of singularity free at workspace is difficult, so finding a best solution that can develop a technique to determine the singularity-free zones in the workspace of parallel manipulators is highly important. in this thesis a simple and new technique are presented to determine the maximal singula...
15 صفحه اولFLOW CYTOMETRY DETERMINATION OF BCl-2 EXPRESSION IN ANIMALS INFECTED WITH BOVINE LEUKAEMIA VIRUS
Investigations were performed in lambs experimentally infected with blood lymphocytes of leukaemic cow. The presence of specific bovine leukaemia virus (BLV) antibodies in sera of infected animals was detected in agar gel immunodiffusion test and ELISA. Proviral DNA was detected with PCR and nested PCR. Dual-colour flow cytometry analysis with the use of specific monoclonal antibodies (for lymp...
متن کاملDetermination of Lymphocyte Subsets and Pcna Activity in Sheep Experimentally Infected with Bovine Leukemia Virus (blv)
Lymphocyte phenotypes were determined in sheep experimentally infected with bovine leukemia virus (BLV). BLV infection was detected by serological tests AGID and ELISA. Flow cytometry analysis with the use of specific monoclonal antibodies for CD markers and conjugates labeled with FITC or PE was performed. Proliferating cell nuclear antigen (PCNA) was estimated in lymphocytes of infected and h...
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ژورنال
عنوان ژورنال: Bulletin of the Veterinary Institute in Pulawy
سال: 2014
ISSN: 2300-3235
DOI: 10.2478/bvip-2014-0055